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IPMA complete metal analysis in seawater samples
UNICA (University of Cagliari) organized a conference the 20 June 2019 in Cagliari
Data Quality Assurance: a holistic view
DGT Conference 2019 will be the fifth international scientific event with MONITOOL presence in 2019
MONITOOL partners share results and plan next steps in Gran Canaria
Technical conferences present MONITOOL project and DGT passive sampling in Canary Islands
Interview with Thi Bolam, leader of MONITOOL Work Package 5
ITC will organize 2 technical conferences in the days preceding the next partners´ meeting in Canary Islands
UNICA promotes the application of the MONITOOL approach for other organic compounds
DCU analyses biofouling on MONITOOL DGTs
Organisation of the interlaboratory DGT exercise by IFREMER, October 2018
Database Management and correlation studies. First promising results.
Interview with Dr. Blánaid White, coordinator of Interreg founded MONITOOL project
MONITOOL partners discuss project´s first results in AZTI, San Sebastián
AZTI hosts 3rd MONITOOL meeting in San Sebastian
DGT extraction and metal analysis by IFREMER
Determination of lead and cadmium labile fraction by Anodic Stripping Voltammetry and total dissolved Nickel Cathodic Stripping Voltammetry
Last EEA report shows the importance of Water Framework Directive (WFD) to keep and improve waters quality in Europe
MONITOOL Project analyses its winter samples and obtains their first results
Sampling and sample processing and analysis WP5 protocols development
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DCU analyses biofouling on MONITOOL DGTs

Biofouling Imagen 1DCU analyses biofouling on MONITOOL DGTs

Passive sampling of contaminants in water bodies is based on the accumulation of target compounds in a membrane, resin or gel, through which water continually flows. The passive samplers used in Monitool  are diffusive gradients in thin-films (DGT), and they are used to monitor metal concentrations in seawaters along the Atlantic coast. Many of the metals we are analysing in Monitool are present in seawaters in very low concentrations. However, DGTs can accumulate these metals in their binding gels so that even very small amounts of metal contaminations can be quantified in marine waters.  One of the critical considerations for deploying DGTs for metal analysis is the length of time for which they will be deployed in the water bodies. In Monitool, we deploy the DGTs for 5 days, but they have also successfully been deployed for longer periods of time.

How effectively the DGT works depends on its capacity to retain the target metal compounds. This  assumes that all the binding sites in the binding gel are available to bind the metal cations. However, this assumption can be compromised by biofouling. Biofouling can be defined as the attachment of flora and fauna to the exposed surfaces of the DGTs. In marine environments, biofouling can be caused by microbial species (e.g. diatoms, bacteria and fungi) and macroorganisms, including mussels, barnacles, seaweed, etc. This biofouling can change the DGT surfaces and this can impact the extent to which the metal cations diffuse through to the binding gel of the DGT, which can potentially reduce the uptake of the metal into the DGT. Conversely, it is also possible that the biofilm which forms as a result of biofouling may increase the metal concentration on the DGTs during deployment, as the biofilm itself may also bind metal cations. Within the Monitool project therefore, it is important to assess the extent to which biofouling occurs on DGTs deployed during the project, and to investigate the potential variations in biofouling between the deployment sites, which range from the Canary Islands to the Scottish Highlands, and in the Mediterranean.

Biofouling assessment template in pristine condition (left) and after deployment (right).

Assessment of biofouling within the Monitool project is being investigated by Dublin City University, the partner coordinating the project. All participating partners deployed additional DGTs in both the wet and dry sampling campaigns, in parallel with the DGTs deployed for the Monitool project, using the same deployment protocols and deployment times. All biofouling DGTs were then returned to DCU for analysis. The DCU analysis includes visualisation of the extent of biofouling using both light microscopy and scanning electron microscopy, followed by evaluation of the biodiversity of the fouling organisms.

In the second Monitool sampling period, custom designed biofouling assessment plates have been designed at DCU and deployed by participating partners, as shown in Figure 2. These plates incorporate multiple materials, including propene sultone, polycarbonate and glass. These materials provide a variety of surfaces for attachment of fouling organisms, which will provide additional data to enable more comprehensive biodiversity assessment. Comprehensive analysis of these plates in conjunction with the deployed DGTS will allow the extent and diversity of biofouling to be evaluated across the Monitool project.